3D Organoid Culture Systems

Human organ and tissue systems are complex and challenging to study.  Current 2D mono-culture cell systems, although quite useful, do not reflect the in vivo organ physiology and level functionality required for organotypic modeling.  Recent advances in three dimensional (3D) culture and co-culture systems in vitro using stem cells and primary progenitors are enabling the development towards spheroids, organoids, organotypic or histotypic organ-on-chip cultures that mimic the in vivo biology of physiological relevance.  Such culture systems open up a new innovative ways to visualize and understand the fundamental complexity of cell biology, and allow for modeling of various tissue disease states.  This approach can shed light on what can go wrong in diseased tissues and offer a powerful tool for drug discovery and toxicity testing.  It is expected that use of such 3D cell culture models will accelerate the progress of research to better understand organ level function­ality, reduce the current error rate in drug discovery, and improve safety in compound testing.  This book provides comprehensive solutions to various 3D cell culture systems modeling main physiological human tissues with detailed but simple to follow protocols.